Superior confocal Raman imaging system

alpha300 R from WITec

The imaging capabilities of the alpha300 R fulfill the highest requirements of confocal Raman imaging with superior performance in speed, sensitivity and resolution. These unique characteristics have established the alpha300 R the preeminent confocal Raman imaging system on the market.

  • Confocal Raman Imaging with unprecedented performance in speed, sensitivity, and resolution
  • Hyperspectral image generation with the information of a complete Raman spectrum at every image pixel
  • Excellent lateral resolution
  • Outstanding depth resolution ideally suited for 3D image generation and depth profiles
  • Ultra-fast Raman imaging option with under one millisecond integration time per spectrum
  • Ultra-high throughput spectroscopic system for highest sensitivity and best performance in spectral resolution
  • Non-destructive imaging technique: no staining of fixation of the sample required

Further information

While long recognized as the state-of-the-art imaging system, ongoing development resulting from WITec's innovative spirit has kept the alpha300 R at the forefront of Raman microscopy and set the benchmark in terms of imaging capability as well as spectral quality, spatial resolution, ease-of-use and compatibility with other measurement techniques.

The flexibility of the alpha300 R series allows the system adapt to all requirements, combine different imaging techniques and to evolve to meet new or expanded needs.

The alpha300 R offers the following extensions:

  • Ultrafast Raman Imaging (1300 spectra per second) optional available
  • Wide choice of lasers and possibility to install additional lasers
  • Dark Field, Phase Contrast and DIC optional
  • Upgradable for epi-fluorescence applications
  • Piezo-driven scan stage (scan range 200 x 200 x 20 µm) (other ranges available)
  • TrueSurface for Raman depth profiling


Raman General Operation Modes:

  • Raman spectral imaging: acquisition of a complete Raman spectra at every image pixel
  • Planar (x-y-direction) and depth scans (z-direction) with manual sample positioning
  • Image stacks: 3D confocal Raman Imaging
  • Time series
  • Single point Raman spectrum acquisition
  • Single-point depth profiling
  • Fibre-coupled UHTS spectrometer specifically designed for Raman microsopy and applications with low light intensities
  • Confocal fluorescence microscopy
  • Bright Field Microscopy

Basic Microscope Features:

  • Research grade optical microscope with 6x objective turret
  • Video system: video CCD camera
  • LED white-light source for Köhler illumination
  • Manual sample positioning in x- and y-direction
  • Fibre coupling

Raman Optional/Upgradable Operation modes

  • Additional lasers, several wavelenghts eligible
  • Additional UHTS-spectrometers (UV, VIS, NIR)
  • Automated, motorized sample positioning and measuring with piezo-driven scan stages
  • Automated confocal Raman imaging
  • Automated multi-area and multi-point measurements
  • Full automation: see alpha300 apyron
  • Ultrafast Raman imaging, optional available
  • Upgradable for epi-fluorescence applications
  • Adapter for higher samples 
  • TrueSurface for Raman depth profiling
  • Autofocus
  • Dark Field Microscopy, Phase Contrast Microscopy, and DIC optional

Ultrahigh-throughput UHTS Spectrometers

  • Various lens-based, excitation optimized spectrometers (UV, VIS or NIR) available, all specifically designed for Raman microsopy and applications with low light intensities
  • Fibre-coupled ultrahigh-throughput optical instruments
  • Superior peak shape conservation

Computer Interface:

  • WITec software for instrument and measurement control, data evaluation and processing


Life Science

From measurements in liquids to solid samples or soft tissues in life science varied samples are analyzed on a regular basis. With their convenient handling and versatile analytical capabilities the flexible WITec imaging systems provide the opportunity to adjust the imaging technique to changing requirements and are particularly well-suited for life science.

Pharmaceutics, cosmetics

The development and production of drug delivery systems requires efficient and reliable control mechanisms to ensure the quality of the final products. These products can vary widely in composition and application. Therefore analytical tools such as the WITec imaging systems that provide both comprehensive chemical characterization and the flexibility to adjust the method to the investigated specimen are preferred in pharmaceutical research.

Materials science

Materials science is a diverse field including the development and testing of new substances, as well as the refinement of manufacturing processes and quality control for existing products. WITec imaging systems are particularly well-suited for comprehensive sample analyses in materials science and provide the opportunity to acquire a thorough knowledge of the sample surface morphology and chemical composition.


WITec confocal Raman imaging systems are excellent analytical tools for the comprehensive investigation of geological samples, such as the identification and characterization of minerals, or in the observation of mineral phase transitions in high and ultra-high pressure/temperature experiments.


WITec imaging systems enable comprehensive sample analysis that provides a thorough characterization of the physical and chemical properties of the polymers on the nanometer scale.

Nano-Carbon & 2D Materials

2D materials such as carbon nano-tubes, graphene or transition metal dichalcogenides (TMDs) show immense promise in many applications such as transistors, sensors, and optoelectronics. Flexible and adaptive analytical methods can support effective investigation and accelerate progress in 2D materials research and development. For comprehensive investigations of nano-carbon and TMD samples WITec microscopes can be equipped with various imaging techniques such as confocal Raman imaging, AFM, and SNOM, all fully integrable in a single microscope.


Spectroscopy solutions brochure
alpha300 high throughput microscope series brochure

Reference customers

"From the initial enquiry, to purchase and installation, the staff at LOT-QuantumDesign were excellent throughout. They gave an excellent demonstration of the alpha 300R+, which helped us to identify the most appropriate components for the research we intended to perform."

- Dr James Bowen at Birmingham University, School of Chemical Engineering

Dr James Bowen has a WITec Confocal Raman Microscope alpha 300R+ system with large area scanning of up to 25mm x 25mm XY. This is available for any collaboration with other research institutions in the UK or for service contract work within industry. Please visit Dr Bowen's website for further information or call Dr James Bowen on +44 121 414 5080, Email The alpha300R+ purchase was funded by ERDF.


Stephane Struyve
Stephane Struyve

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